General Cell Based Assays
Eurofins Discoverx PathHunter eXpress F2RL1 (PAR2; GPR11) U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0235E3CP0M)
The PathHunter F2RL1 (PAR2; GPR11) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL1 and EA-tagged β-Arrestin-2. Activation of F2RL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 4 Reagent, 500. (93-0563R4B)
AssayComplete Cell Plating 4 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress PRLR-JAK2 Functional Assay, 2-Plate Kit. (93-0687E3CP0M)
The PathHunter PRLR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses PRLR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PRLR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0859E3CP0M)
The PathHunter CHRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM1 and EA-tagged β-Arrestin-2. Activation of CHRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MC1R U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0861E3CP5L)
The PathHunter MC1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC1R and EA-tagged β-Arrestin-2. Activation of MC1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL6RA/6ST Bioassay Kit, 2-Plate. (93-1045Y3-00043)
The PathHunter IL6RA/IL6ST Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-6-induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6R target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx KILR Detection Kit, 2,500 dp (96-well). (97-0001L)
The KILR Detection Kit is a detection kit intended for use with KILR cell lines or pools when running ADCC or other lysis-based assays. This kit contains exogenous EA as well as EFC substrate to allow for detection of lysis-based release of ProLabel-tagged KILR reporter protein. This kit contains all components required to assay 25 plates (96-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-97-0001l.
Eurofins Discoverx cAMP Hunter eXpress CALCRL-RAMP1 CHO-K1 GPCR Assay, 2pl Kit. (95-0164E2CP2M)
The cAMP Hunter CALCRL-RAMP1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-CGRP) based activation of CALCRL (paired with RAMP1) GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL, a naturally Gs coupled GPCR, alongside modulator protein RAMP1. Upon activation of CALCRL, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems QuantiChrom™ Phytase Assay Kit. A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples.
A simplest, direct "Add-and-Measure" assay for phytase enzyme activity in agricultural and biological samples. Key Features: Simple and convenient: No complex detection reagents to mix. High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay. Fast and high-throughput: Homogeneous "mix-and-measure" assay allows for quantification of phytase activity within 60 minutes. Method: OD620nm. Samples: Enzyme extracts, agriculture and biological samples. Species: All (e.g. Wheat, Barley). Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.01 U/L.
Bioassay Systems EnzyChrom™ NADP/NADPH Assay Kit. For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts.
For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts. Key Features: Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: Colorimetric Determination at 565 nm. Samples: Cells, Tissue. Species: human, mouse, rat and more. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 µM.
Sigma Aldrich Fine Chemicals Biosciences ATPase/GTPase Activity Assay Kit sufficient for 200 colorimetric tests | 1KT
ATPase/GTPase Activity Assay Kit sufficient for 200 colorimetric tests | 1KT
Abcam DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates)
DCF ROS/RNS Assay Kit (biofluids, culture supernatant, cell lysates)
The product is subject to the following: Abcam Restricted Use Statement
Abcam DHE (Dihydroethidium) Assay Kit - Reactive Oxygen Species
DHE (Dihydroethidium) Assay Kit - Reactive Oxygen Species
The product is subject to the following: Abcam Restricted Use Statement
Aat Bioquest Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*
Mitochondria are major producers of cellular superoxide.