General Cell Based Assays
Eurofins Discoverx PathHunter eXpress BMPR1A/ACVR2 Dimerization Assay, 10pl Kit. (93-1037E3CP5L)
The PathHunter BMPR1A/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. BMP-4) induced dimerization of BMPR1A/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses BMPR1A target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of BMPR1A/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR109A CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0301E2CP2L)
The PathHunter GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109A and EA-tagged β-Arrestin-2. Activation of GPR109A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR135 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0431E2ACP1L)
The PathHunter GPR135 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR135 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR135 and EA-tagged β-Arrestin-2. Activation of GPR135 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY8 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0635E2CP1L)
The PathHunter P2RY8 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY8 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY8 and EA-tagged β-Arrestin-2. Activation of P2RY8 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mEDNRB CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0602E2MCP0L)
The PathHunter mEDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of mEDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRB and EA-tagged β-Arrestin-2. Activation of mEDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0629E2CP2M)
The PathHunter CCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL13/MCP-4) based activation of CCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR3 and EA-tagged β-Arrestin-2. Activation of CCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Adipogenesis Assay Kit (Cell-Based)
Adipogenesis Assay Kit (Cell-Based)
The product is subject to the following: Abcam Restricted Use Statement
Abcam beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric)
beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Glucokinase Activity Assay Kit (Fluorometric)
Glucokinase Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Glucose Assay Kit (Fluorometric, High Sensitivity)
Glucose Assay Kit (Fluorometric, High Sensitivity)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical DuP-697 Assay Reagent, CAS: 88149-94-4, 1 ea
DuP-697 Assay Reagent, CAS: 88149-94-4, 1 ea
Cayman Chemical CelBased asy NBD CholSte 500uL
A DYRK1A inhibitor (IC50 0.4 nM) selective for DYRK1A over DYRK2 Clk1 Clk2 Clk3 Cdk2 and GSK3B (IC50s 19 7.1 9.4 54 100 and 94 nM respectively) but does inhibit DYRK1B (IC50 2.7 nM) inhibits DYRK1A-induced increases in tau phosphorylation in a cell-based assay (IC50 590 nM)
Aat Bioquest Screen Quest™ Fluo-8 No Wash Calcium Assay Kit | 10 Plates
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest™ Fluo-8 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Fluo-8 NW which can cross cell membrane. Fluo-8 NW is the brightest calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Fluo-8 AM are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-8 NW.
Revvity Health Sciences Inc LANCE Ultra Phospho-SMAD2 (Ser465/467) Detection Kit, 500 Assay Points
LANCE Ultra Phospho-SMAD2 (Ser465/467) Detection Kit, 500 Assay Points